Problem Possible Cause Solution No Signal Inactive enzyme on secondary antibody Test enzyme activity by luminometer or dotting secondary antibody directly to membrane. AP enzyme should never be frozen; avoid HRP freeze-thaw cycles. Primary or secondary antibody no longer bind to target Avoid antibody freeze-thaw cycles; use a fresh aliquot of antibody. Make sure correct antibodies were used and Read More :-

Choosing Substrate AP vs HRP

Choosing the right substrate for chemiluminescent Western blotting may be a daunting task, given the multitude of options available. The key factors to consider are the level of sensitivity desired, the reporter enzyme used, cost of reagents, versatility, and the nature of the experiment to be performed. One useful starting point in selecting the right chemiluminescent substrate is deciding which Read More :-

New Products from Giri Diagnostic Pvt.Ltd

Giri Diagnostic Pvt.Ltdis proud to introduce two new products in our lineup: diagnostic testing kits and our antibody/conjugate line. Our production process results in the most reliable, consistent, highest quality products the industry has to offer. We are confident that these new products will provide the same kind of results that we have come to be known for. Our Read More :-

Giri Diagnostic Pvt.Ltd’s, New Buffer Line up

Giri Diagnostic Pvt.Ltd’s, New Buffer Line up Giri Diagnostic Pvt.Ltdis proud to introduce a line of high quality buffers for various applications. These include: prewash, wash, blocking, coating and enzyme stabilizing buffers. All buffers contain high quality reagents and preservatives to ensure optimal performance and stability. For more flexibility and added stability we also offer powdered buffers, which are Read More :-

Giri Diagnostic Pvt.Ltd’s, Highly Sensitive Chemiluminescent Reagents

Giri Diagnostic Pvt.Ltd’s, Highly Sensitive Chemiluminescent Reagents For years Giri Diagnostic have made some of the best Chemiluminescent Reagents for the detection of Alkaline Phosphatase (AP) and Horseradish Peroxidase (HRP) in the market. Their patented formulas offer detection levels that cannot be matched by their competition. Horseradish peroxidase enzyme is the most widely used in EIA and EIH. Typical peroxidases are hemoproteins Read More :-